eEF1A is an S-RNase binding factor in self-incompatible Solanum chacoense.

eEF1A is an S-RNase binding factor in self-incompatible Solanum chacoense.

Blog Article

Self-incompatibility (SI) is a genetic mechanism that allows flowering plants to identify and block fertilization by self-pollen.In the Solanaceae, SI is controlled by a multiallelic S-locus encoding both robocop tshirt S-RNases and F-box proteins as female and male determinants, respectively.S-RNase activity is essential for pollen rejection, and a minimum threshold value of S-RNases in the style is also required.Here we present biochemical evidence that eEF1A is a novel S-RNase-binding partner in vitro.

We further show that the normal actin binding activity of eEF1A is enhanced by the presence of S-RNase.Lastly, we find that there is a co-localization of S-RNase and actin in the incompatible pollen tubes in structures reminiscent of the actin bundles formed by eEF1A.We propose that increased binding of eEF1A to actin in the presence of S-RNase could help explain the disruption of funky chunky chocolate pretzels the actin cytoskeleton observed during SI reactions.